Pipeline to Identify Hydroxyproline-Rich Glycoproteins

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Breakthrough Technologies A Motif and Amino Acid Bias Bioinformatics Pipeline to Identify Hydroxyproline-Rich Glycoproteins

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A bioinformatics approach to the identification, classification, and analysis of hydroxyproline-rich glycoproteins.

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Localization of soluble and insoluble fractions of hydroxyproline-rich glycoproteins during maize kernel development

Monoclonal antibodies have been developed against PC-1 protein, the major hydroxyproline-rich glycoprotein (HRGP) from maize pericarp, and have been used as probes in an indirect alkaline phosphatase antibody assay to localize this protein in tissue of three maize varieties. Kernels were sampled several days after pollination (d.a.p.). HRGPs were assayed by reacting antibodies with tissue print...

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Biochemical and tissue print analyses of hydroxyproline-rich glycoproteins in cell walls of sporophytic maize tissues.

In an effort to understand the role of hydroxyproline-rich glycoproteins (HRGPs) in plant cell wall structure, we studied the distribution and physical properties of PC-1-like proteins (PC-1 being the major pericarp HRGP) throughout sporophytic tissues of two maize (Zea mays L.) varieties. We determined total amounts of hydroxyproline, an indicator of HRGPs, and did tissue print and Western blo...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 2017

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.17.00294